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Promega ks vector primer
Ks Vector Primer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ks+vector+primer/ks+vector+primer/10__1074_slash_jbc__274__45__31853-121-20-23
Average 90 stars, based on 1 article reviews
ks vector primer - by Bioz Stars, 2026-09
90/100 stars

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Control:

Article Title: Expression of the Gene for Mitoribosomal Protein S12 Is Controlled in Human Cells at the Levels of Transcription, RNA Splicing, and Translation
Article Snippet: .. For the control rpL22 transcript used in RT-PCR assays, the primers were rpL22 (CGTGGGCACGTCAGTCAC) (np 57–75, Ref. 44) and the KS vector primer (Promega), which amplify a 600-bp PCR product encompassing the cloned cDNA and a small portion of the pBSK plasmid (Promega), thus avoiding the amplification of the endogenous rpL22 mRNA. .. Probes for Northern analysis of RPMS12 mRNAs were derived by PCR amplification of a full-length RPMS12 cDNA clone and were gel-purified using the QIAquick gel extraction kit (Qiagen) before radiolabeling.

Reverse Transcription Polymerase Chain Reaction:

Article Title: Expression of the Gene for Mitoribosomal Protein S12 Is Controlled in Human Cells at the Levels of Transcription, RNA Splicing, and Translation
Article Snippet: .. For the control rpL22 transcript used in RT-PCR assays, the primers were rpL22 (CGTGGGCACGTCAGTCAC) (np 57–75, Ref. 44) and the KS vector primer (Promega), which amplify a 600-bp PCR product encompassing the cloned cDNA and a small portion of the pBSK plasmid (Promega), thus avoiding the amplification of the endogenous rpL22 mRNA. .. Probes for Northern analysis of RPMS12 mRNAs were derived by PCR amplification of a full-length RPMS12 cDNA clone and were gel-purified using the QIAquick gel extraction kit (Qiagen) before radiolabeling.

Plasmid Preparation:

Article Title: Expression of the Gene for Mitoribosomal Protein S12 Is Controlled in Human Cells at the Levels of Transcription, RNA Splicing, and Translation
Article Snippet: .. For the control rpL22 transcript used in RT-PCR assays, the primers were rpL22 (CGTGGGCACGTCAGTCAC) (np 57–75, Ref. 44) and the KS vector primer (Promega), which amplify a 600-bp PCR product encompassing the cloned cDNA and a small portion of the pBSK plasmid (Promega), thus avoiding the amplification of the endogenous rpL22 mRNA. .. Probes for Northern analysis of RPMS12 mRNAs were derived by PCR amplification of a full-length RPMS12 cDNA clone and were gel-purified using the QIAquick gel extraction kit (Qiagen) before radiolabeling.

Polymerase Chain Reaction:

Article Title: Expression of the Gene for Mitoribosomal Protein S12 Is Controlled in Human Cells at the Levels of Transcription, RNA Splicing, and Translation
Article Snippet: .. For the control rpL22 transcript used in RT-PCR assays, the primers were rpL22 (CGTGGGCACGTCAGTCAC) (np 57–75, Ref. 44) and the KS vector primer (Promega), which amplify a 600-bp PCR product encompassing the cloned cDNA and a small portion of the pBSK plasmid (Promega), thus avoiding the amplification of the endogenous rpL22 mRNA. .. Probes for Northern analysis of RPMS12 mRNAs were derived by PCR amplification of a full-length RPMS12 cDNA clone and were gel-purified using the QIAquick gel extraction kit (Qiagen) before radiolabeling.

Clone Assay:

Article Title: Expression of the Gene for Mitoribosomal Protein S12 Is Controlled in Human Cells at the Levels of Transcription, RNA Splicing, and Translation
Article Snippet: .. For the control rpL22 transcript used in RT-PCR assays, the primers were rpL22 (CGTGGGCACGTCAGTCAC) (np 57–75, Ref. 44) and the KS vector primer (Promega), which amplify a 600-bp PCR product encompassing the cloned cDNA and a small portion of the pBSK plasmid (Promega), thus avoiding the amplification of the endogenous rpL22 mRNA. .. Probes for Northern analysis of RPMS12 mRNAs were derived by PCR amplification of a full-length RPMS12 cDNA clone and were gel-purified using the QIAquick gel extraction kit (Qiagen) before radiolabeling.

Amplification:

Article Title: Expression of the Gene for Mitoribosomal Protein S12 Is Controlled in Human Cells at the Levels of Transcription, RNA Splicing, and Translation
Article Snippet: .. For the control rpL22 transcript used in RT-PCR assays, the primers were rpL22 (CGTGGGCACGTCAGTCAC) (np 57–75, Ref. 44) and the KS vector primer (Promega), which amplify a 600-bp PCR product encompassing the cloned cDNA and a small portion of the pBSK plasmid (Promega), thus avoiding the amplification of the endogenous rpL22 mRNA. .. Probes for Northern analysis of RPMS12 mRNAs were derived by PCR amplification of a full-length RPMS12 cDNA clone and were gel-purified using the QIAquick gel extraction kit (Qiagen) before radiolabeling.



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